課程名稱 |
蛋白體與後修飾之質譜分析 Mass Spectrometry-based Proteomics and Protein Modification Analysis |
開課學期 |
106-2 |
授課對象 |
醫學院 基因體暨蛋白體醫學研究所 |
授課教師 |
邱繼輝 |
課號 |
BChem5027 |
課程識別碼 |
B46 U0840 |
班次 |
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學分 |
2.0 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
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上課地點 |
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備註 |
本課程中文授課,使用英文教科書。上課地點為中研院生化所409室,上課時間:每週一下午4:00~6:00。 總人數上限:30人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1062BChem5027_ |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
106(2)為中文上課,請外籍學生於107(2)再行修課。
The course this semester will be taught in Chinese, foreign students please take this course next year
This course aims to teach the fundamentals of biological mass spectrometry with particular focus on applications to proteomics and analysis of protein modifications. The course will begin with an introduction to the general aspects of mass spectrometry-based peptide sequencing and protein identification, along with recent technological developments in the context of advancing quantitative shotgun proteomic analysis. Current perspectives in global versus directed analysis, speed versus depth in proteomic coverage, will be discussed. This will then be followed by introducing the practical aspects of MS analysis of select protein modifications, at both the levels of single targeted protein of interest and the proteomic scale. Topics include phosphoproteomics; lysine modifications such as acetylation, methylation, ubiquitination, and SUMOylation; cysteine modifications such as oxidation, glutathionylation and S-nitrosylation; and glycosylation. The last further accompanied by an overview of MS-based glycomics and glycoproteomics. |
課程目標 |
待補 |
課程要求 |
Opens to life science Master and PhD Students, as well as keen senior undergraduates major in Biochemistry, who have a basic knowledge of protein chemistry and structures, and the commonly found modifications. No absolute prerequisite is set but will be advantageous and encouraged to have first taken one or more of the core courses offered at 生化所 in the first semester eg 蛋白質修飾機制與細胞訊息;結構生化學. Chemistry graduates interested in biology and major in chemical biology are also encouraged and accepted. |
預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
待補 |
參考書目 |
待補 |
評量方式 (僅供參考) |
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週次 |
日期 |
單元主題 |
第1週 |
2/26 |
因教師出國,停課一次
no class |
第2週 |
3/5 |
Overview on DDA & Technical Evolu;on of MS-based Proteomics I |
第3週 |
3/12 |
DDA & Technical Evolu;on of MS-based Proteomics II |
第4週 |
3/19 |
Quan;ta;ve Shotgun Proteomics |
第5週 |
3/26 |
From Global Discovery to Targeted Proteomics, SRM/PRM & DIA |
第6週 |
4/2 |
溫書假 Holiday, no class, reading and preparing for seminars |
第7週 |
4/9 |
Quantitative Proteomics to probe Spaiotemporal Proteome Dynamics |
第8週 |
4/16 |
Proteome Network, Complexes & Interactomics |
第9週 |
4/23 |
Proteomics Seminars I |
第10週 |
4/30 |
Proteomics Seminars II |
第11週 |
5/7 |
PTM - General Technical Considera;ons; Phospho |
第12週 |
5/14 |
No class, reading and preparing for seminars |
第13週 |
5/21 |
PTM - Lys Modifica;ons : Ub/SUMO, Ac/Me |
第14週 |
5/28 |
PTM - Glycosyla;on |
第15週 |
6/4 |
PTM - Seminars I |
第16週 |
6/11 |
PTM - Seminars II |
第17週 |
6/18 |
端午節 Holiday, no class |
第18週 |
6/25 |
Final Recap with Q&A Oral Exam |
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